Scientific Series: Red Cell Genotyping in the Immunohematology Lab

Speaker(s): 

Dr. Phil Berardi

Objectives:

1. Background and timeline of blood group genotyping.

2. Review ‘common’ causes of variability in RhD antigen expression and detection.

3. Outline some challenges of typing for RhD/RhCE by serologic methods.

4. Indications for RBC genotyping in patients likely to require transfusion.

5. Review of interesting clinical cases.

References:

1. Transfusion. 2015 Mar; 55(3): 680–689.

It's time to phase in RHD genotyping for patients with a serologic weak D phenotype.

S. Gerald Sandler, Willy A. Flegel, Connie M. Westhoff, Gregory A. Denomme, Meghan Delaney, Margaret A. Keller, Susan T. Johnson, Louis Katz, John T. Queenan, Ralph R. Vassallo and Clayton D. Simon

2. Lancet Haematol. 2015 Jul;2(7):e282-9.

Integration of red cell genotyping into the blood supply chain: a population-based study.

Flegel WA, Gottschall JL, GA. Denomme

3. Blood. 2013;122:1062-71

High prevalence of red blood cell alloimmunization in sickle cell disease despite transfusion from Rh-matched minority donors.

Chou ST, Jackson T, Vege S, Smith-Whitley K, Friedman DF, CM. Westhoff

4. Blood. 2009 Jul 9;114(2):248-56. doi: 10.1182/blood-2008-11-146860. Epub 2009 May 1.

Red cell genotyping and the future of pretransfusion testing.